FastQCFastQC Report
Sun 19 Mar 2023
SRR4896131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790922
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG238683.0177438483187977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG61650.7794700362361902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58620.7411603166936815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC31540.3987751004523834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA31180.39422345060574876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT472100.0113.902431
GGTATCA484700.0111.152748
GTATCAA485750.0110.890559
AGCAGTG487200.0110.283812
AGTGGTA485850.0110.2549445
GTGGTAT486100.0110.176386
TGGTATC487250.0110.005617
CAGTGGT489150.0109.577354
GCAGTGG494650.0108.461573
CGTAATA805.8739533E-754.37256145
AGCGGTA1755.2750693E-1141.431955
GCGGTAT1755.2750693E-1141.4293336
GGGTATC3200.040.7819987
CGACTGT1105.26453E-639.54368145
CGGTATC1859.640644E-1139.189917
AGGTATC3500.035.214937
AGGGGTA3950.033.040675
GAGGTAT4100.030.0615276
AAATATA28400.029.866617145
AGAGGTA4600.028.3718785
Produced by FastQC (version 0.11.5)