FastQCFastQC Report
Sun 19 Mar 2023
SRR4896130_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896130_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220693
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30501513.735126827526361No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC479762.160406683859498No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA415831.8725235770995812No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATA383601.72738870253565No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATA357371.6092724208163847No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGATA214620.9664550660537048No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTTGATA174720.7867814236366755No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCATGTACTCTGCGTTGATA164550.7409849087649666No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA163860.73787777058783No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCTGATA139200.6268313539962526No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATA129490.5831062645759679No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA126150.5680659145591038Illumina Single End PCR Primer 1 (100% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA120510.5426684372851177No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATA103630.46665612941545725No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCAGGTACTCTGCGTTGATA95880.43175711365776354No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATA92470.41640154672437835No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGCGCGGCGGGTA90350.40685497725259634No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGGGTA85320.38420439025115133No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACGCTGCGTTGATA82670.3722711784114238No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCGGATA82050.3694792571508083No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCATGTACTCTGCGTTGATA79780.3592572228579097No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCATGTACTCTGCGTTGATAC79650.3586718200129419No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGTTGATA74340.3347603653454124No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA70490.31742343493675174No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGCGTTGATA69430.31265015020086073No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACTCTGCGTTGATA61800.2782915063000604No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCTGATA56780.2556859502866898No Hit
AGATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGTTGATA55960.2519934092645854No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGTACTCTGCGTTGATA54200.2440679553634834No Hit
AGATGTACTCTGCGCTGATACCACTGCTTCCCATGTACTCTGCGTTGATA52780.23767355505691243No Hit
AGATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA51970.2340260450228825No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGGACTCTGCGTTGATA46940.21137545802143745No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGTACTCTGCGTTGATAC46490.20934906355808752No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCGGCGCGGATA41910.1887248710199924No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACGCTGCGTTGATA41380.1863382286520469No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGTGTA40980.18453698912906918No Hit
AGATGTACTCTGCGTTGATACCACTGCAACCCATGTACTCTGCGTTGATA40310.18151991292808145No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCAGGTACTCTGCGTTGATA39310.1770168141206371No Hit
AGATGTACTCTGCCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA38170.17188328148015056No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGGTA36270.1633273937460063No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACGCTGCGTTGATA34310.1545013200834154No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCTGCGCTGATA31790.14315351108865565No Hit
AGATGTACTCTGCGTCGATACCACTGCTTCCCATGTACTCTGCGTTGATA30970.1394609700665513No Hit
AGATGTACTCTCCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30840.13887556722158353No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGACGCGCGGCGGGTA29730.1338771275453203No Hit
AGATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA29270.13180570209389592No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGTACTCTGCGTTGATA28990.1305448344278115No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCACGTACTCTGCGTTGATA28440.1280681300837171No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTGGATA28200.12698738636993048No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCATGTACTCTGCGTTGATAC27590.12424049609738941No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACGCTGCGTTGATA27420.12347496930012389No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGGTGAAAC27000.12158366780099725No Hit
AGATGTACTCTGCGTTGATACCACTGCGTAGATCGGAAGAGCGTCGTGTA26290.11838646764771178Illumina Single End PCR Primer 1 (100% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTTGATA26150.11775603381466955No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGCTGATA26100.11753087887429733No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACTCTGCGTTGATA26000.1170805689935529No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCATGTACTCTGCGTTGATAC25970.11694547602932959No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGTCGTGTA25760.11599982527976628Illumina Single End PCR Primer 1 (95% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGGACTCTGCGTTGATA25560.11509920551827739No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGTTGATA24610.11082126165120527No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGCTGATA24520.11041598275853529No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTGGATA23740.1069035656887287No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAGA23500.10582282197494206No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATGGGCAGAGCGTCGAGAC23100.10402158245196433No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCTGCGTTGATA22980.103481210595071No Hit
AGATGTACTCTGCGTTGATACCACTGCGGCCCATGTACTCTGCGTTGATA22490.10127469217942327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGTA2222850.0144.751341
GATGTAC2253850.0142.760382
TGTACGT76900.0142.539494
TGTACGA61250.0142.529114
GTACGTG26450.0142.429615
TGTACAT59650.0140.886754
GTACTTG26500.0140.793955
GTACTGA16950.0140.620565
TGTACGG76050.0140.512654
GTACGTT23950.0140.357335
TACGGTA7050.0139.756626
TGTACTT78950.0138.930114
GTACGAT21800.0138.580525
TGTACAA48100.0138.267554
CGATTAG1050.0137.994988
GTACGTA22450.0137.795215
TGTACTA59050.0137.778824
TGTACTG76850.0137.730134
GTACAAT16850.0137.585515
GTACATG20000.0137.287775
Produced by FastQC (version 0.11.5)