FastQCFastQC Report
Sun 19 Mar 2023
SRR4896129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926468
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCG294800.0144.68773
CAGATTC928000.0144.666852
ACAGATT928300.0144.620121
AGATTCA279850.0144.158073
GATTCGA85450.0144.145774
GATTCAG93400.0144.133164
GATTCGG91850.0143.646774
GATTCGT115800.0143.407264
AGATTCT329800.0142.693
GATTCTG106200.0142.316364
GATTCAT105500.0142.299164
ATTCGGA24000.0142.191865
ATTCGAG26050.0141.849415
ATTCGGT29350.0141.452825
ATTCGTA37200.0141.01355
GATTCAA81850.0140.748414
GATTCTA100750.0140.234424
ATTCGTG33900.0140.206855
ATTCGAT31600.0139.635335
ATTCAGG33100.0139.217625
Produced by FastQC (version 0.11.5)