FastQCFastQC Report
Sun 19 Mar 2023
SRR4896129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926468
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG312713.375291969069628No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72540.7829736159262921No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43610.47071242611725395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35350.38155662149151404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18300.19752436133789833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA12570.13567656950914658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCGCAGTGGCTCA11650.12574638303751454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11080.11959398489748163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10460.11290190271007741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGTGGTGGCTCATG9590.10351140028581668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT559750.0107.4199141
GTATCAA573650.0105.2733469
GGTATCA574850.0105.014478
AGTGGTA570600.0104.9585
AGCAGTG573350.0104.7946852
GTGGTAT575400.0104.019446
CAGTGGT576000.0103.997834
TGGTATC577150.0103.766847
GCAGTGG587350.0102.086933
AGGGGTA4200.063.8733525
AGCGGTA1950.055.7729875
CGGTATC2150.053.957127
CGGTCTG1301.3096724E-1050.19027145
GCGGTAT2200.049.4351466
GGGGTAT5550.048.336596
CATACGA450.0096404648.33137145
GGGTATC5800.046.2531137
TCGTTTA1002.7346869E-643.498234145
CAGGGGT6450.041.596444
AGGTATC4200.037.978757
Produced by FastQC (version 0.11.5)