FastQCFastQC Report
Sun 19 Mar 2023
SRR4896128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107956
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3500332.42339471636593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA49764.609285264366965No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7190.6660120789951461No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTCGTGTAGATCGGAAGAGCAC5260.48723554040535033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAGTGGTATCAACGCAG5050.4677831709214865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA4140.3834895698247434No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3940.36496350364963503No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3120.28900663233169066No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3030.2806699025528919No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2680.24824928674645227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2570.23805995035014266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.22972322057134387No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2390.22138649079254513No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2350.21768127755752345No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2320.2149023676312572No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2280.2111971543962355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2100.19452369483863796No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2060.1908184816036163No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2050.18989217829486088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT2030.18803957167735003No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2010.1861869650598392No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1990.18433435844232834No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1970.18248175182481752No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1930.17877653858979586No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1910.176923931972285No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1860.1722924154285079No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1850.1713661121197525No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1780.16488198895846456No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1750.1621030790321983No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1740.16117677572344288No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1730.16025047241468746No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1700.1574715624884212No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1690.15654525917966577No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1600.148208529400867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGTGTAGATCGGAAGAGCA1550.14357701285708993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1490.13801919300455742No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT1460.13524028307829117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1420.1315350698432695No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1390.1287561599170032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTCCATCAGTTGTCCGTA1300.12041943013820446No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1300.12041943013820446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1240.11486161028567193No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1230.1139353069769165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGCTCGAGATCTGCAGT1220.1130090036681611No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT1150.10652488050687317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTCG100.007073169145.061635
AGTCACC151.2271792E-4144.99445145
TTATCTA100.0070829773144.994459
CAGAGCC406.63758E-572.49722145
CATGCGT350.00355843462.140472145
CAGGGGG400.00603724854.372913145
GACTCAA400.00603724854.372913145
CGTGGGG1203.5706762E-948.33148145
CGGGGGG450.00961749548.33148145
CCCGGCT901.3128829E-648.33148145
AAGCAGT197500.047.337831
AGCAGTG208600.044.888442
AGTGGTA210950.044.388385
GTATCAA214150.043.907969
CAGTGGT214900.043.572494
GCCGGCT1505.275069E-1043.498333145
GCAGTGG216550.043.240493
GGTATCA217400.043.2182168
TGGTATC217750.042.9489867
GTGGTAT218100.042.846826
Produced by FastQC (version 0.11.5)