FastQCFastQC Report
Sun 19 Mar 2023
SRR4896127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624695
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT79321.2697396329408752No Hit
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCGGCGTTGATACCACT10290.164720383547171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC625600.0144.690542
AATGTTG626850.0144.402021
TGTTGCA170950.0143.935763
TGTTGCG236100.0143.897873
GTTGCGA62100.0143.277154
GTTGCAG57150.0143.135654
GTTGCAT61350.0143.020974
TTGCGAT21650.0142.567925
GTTGCGT95850.0142.567234
TGTTGCT203300.0142.558383
GTTGCAA52600.0142.293384
GTTGCGG78050.0141.475814
GTTGCTG73150.0141.344774
TTGCTAG20400.0141.00375
GTTGCTA59500.0140.77034
TTGCTGA19850.0140.530435
TTGCGAA18000.0140.080235
TTGCGTA19900.0139.813235
TTGCAGT20300.0138.129065
TTGCGAG20200.0138.095495
Produced by FastQC (version 0.11.5)