FastQCFastQC Report
Sun 19 Mar 2023
SRR4896126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207888
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22291.0722119602863078No Hit
AATCCGTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2940.14142230431770955No Hit
AATCCGTCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT2740.13180173939813744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG100.0070958217144.938649
ATCGATC100.0070958217144.938649
CTAGATG151.2318678E-4144.903788
GTCGCAT202.1336327E-6144.903786
CGTATAG100.0071009286144.903788
GTCTGAT2200.0144.903786
CAGTTGA253.7156497E-8144.903788
CTATAGT253.7156497E-8144.903788
TCGGGAT550.0144.903787
TCAGCAG100.0071009286144.903787
GTCGTCA400.0144.903786
GTCAAGC450.0144.903786
CGAGATT100.0071009286144.903788
TCGACGT151.2318678E-4144.903787
GTCGTAC351.0913936E-11144.903766
ATCCGTC208050.0144.764482
AATCCGT208150.0144.694931
TCCGTCA63200.0144.10133
CCGTCAG20150.0143.825094
CCGTCAT27000.0143.562074
Produced by FastQC (version 0.11.5)