FastQCFastQC Report
Sun 19 Mar 2023
SRR4896126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207888
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG196659.459420457169244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28921.3911336873701223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28331.3627530208573848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA21841.0505656892172708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10030.4824713307165397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAGATTTATAGGTAGAG8310.3997344724082198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5990.2881359193411837No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5170.24869160317093822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4890.2352228122835373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG4230.20347494804894944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT3430.16499268837066114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCTGTCTCTA3220.15489109520511046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG2710.13035865466020166No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2590.1245863157084584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCAACTTTAAATTTGCC2320.1115985530670361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2210.10630724236127145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2140.10294004463942123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTCTACTAAAAATACAAA2110.10149695990148541No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATCTTAGTTCAACTTTA2080.1000538751635496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG2080.1000538751635496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT228850.096.794021
AGTGGTA234200.094.312435
AGCAGTG235950.093.797332
CAGTGGT236200.093.575254
GTATCAA236500.093.3727659
GGTATCA237700.093.076278
GTGGTAT237400.092.9189766
TGGTATC238350.092.548627
GCAGTGG240850.091.828853
GGTTAGT406.6520435E-572.49724145
CGCAAGG300.001933870772.49723145
TTACGGA702.3260873E-762.140484145
TGATTGG805.8525256E-754.372925145
GATATAG953.1241143E-853.419014145
AGCGATG450.00962993148.33149145
GACCCAT1109.9113095E-846.134605145
CTTGTAA8350.044.279747145
GGGTATC1351.0175427E-842.971667
AGCAGGG1351.0175427E-842.971662
CCTTATT700.001054906641.42699145
Produced by FastQC (version 0.11.5)