FastQCFastQC Report
Sun 19 Mar 2023
SRR4896125_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896125_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855643
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTG857050.0144.678312
CAGATCT857100.0144.669881
GATCTGG306150.0144.213443
GATCTGA221650.0144.204283
GATCTGT281400.0143.996483
ATCTGAG86600.0143.584724
ATCTGTG116850.0142.877094
ATCTGGA80050.0142.841524
ATCTGGT107150.0142.827094
ATCTGGG117800.0142.4634
ATCTGAT74100.0141.403094
ATCTGTA79100.0141.25924
ATCTGAA61100.0141.247044
GATCTGC52300.0140.7673
TCTGGAT26200.0139.945225
TCTGAGT25900.0139.887565
TCTGAAG19150.0139.626045
TCTGATG28450.0138.810755
ATCTGTT87300.0138.532264
TCTGAGA21350.0138.474935
Produced by FastQC (version 0.11.5)