FastQCFastQC Report
Sun 19 Mar 2023
SRR4896125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855643
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG349144.080440090084299No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG95231.1129641684674567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA50360.5885632208759961No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46930.5484764089696287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43230.5052340754263168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA11690.13662239976251778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9830.11488436181912316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGGTGCAGTGGCTTAT9550.1116119690104401No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT9060.10588528159524474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT336150.083.167591
AGCAGTG344450.081.1493152
GTATCAA344500.081.102289
GGTATCA344850.081.019968
GTGGTAT347500.080.047456
CAGTGGT348800.079.8462454
TGGTATC350350.079.4376757
AGTGGTA351250.079.2227555
GCAGTGG360950.077.258973
AAATATA30050.047.043613145
CGCTGTT3201.8189894E-1229.451262145
GGGGTAT3357.4578566E-1125.9685066
GAAACCG18550.023.99634685-89
AAACCGC18450.023.96923385-89
CATGGGG75350.023.57293525-29
GCATTGA19500.022.82889630-34
CGCAGAT19700.022.74407890-94
GCAGATA19900.022.3697690-94
ACAACTA20250.022.127111130-134
CTACTTA20000.022.111877135-139
Produced by FastQC (version 0.11.5)