FastQCFastQC Report
Sun 19 Mar 2023
SRR4896124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436348
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG100.0070988243144.936289
ACTGACT306.530172E-10144.919667
AGGACAC438150.0144.307772
AAGGACA438900.0144.061171
GGACACT163550.0143.989273
GACACGT45500.0143.964164
GGACACG129000.0143.852433
GACACTG54250.0143.717584
GACACTT59800.0143.707984
GACACAT46400.0143.201874
GGACACA130050.0142.690983
GACACTA48550.0142.680944
GACACGA39050.0142.136324
GACACGG43950.0141.292564
ACGTTAG1900.0141.105997
ACACGTT15550.0140.725855
GACACAG43350.0140.573764
ACACTTG19400.0139.690615
ACACTGA15950.0139.468145
ACACTGT18200.0138.94775
Produced by FastQC (version 0.11.5)