FastQCFastQC Report
Sun 19 Mar 2023
SRR4896124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436348
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG177484.067395748347649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58691.3450273634805248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24260.5559782558875026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG9490.2174869599493982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA9030.20694491552613967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA7120.16317251368174027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA6710.1537763436523142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6010.13773410213865997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAACATTTTATGTCACTAT6010.13773410213865997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT5750.13177555529073126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTGATGTGTTTGATAATT5410.12398360941267063No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5170.11848341232227488No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4790.1097747669291483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGAAGAAATGCAAACTAT4790.1097747669291483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA4540.10404539495998608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT344200.0114.691371
AGTGGTA350550.0112.187235
GGTATCA353150.0111.874558
GTATCAA353500.0111.833019
AGCAGTG353650.0111.449852
CAGTGGT353850.0111.161454
GTGGTAT353900.0111.0638056
TGGTATC355000.0110.84227
GCAGTGG357850.0109.898663
AGGGGTA1850.054.8693735
AGCGGTA1152.4774636E-950.438935
TAGTGCG450.00963675648.331768145
GGGTATC2150.047.213187
CGGTATC1255.180482E-946.4038127
ACGTTTC1354.9640767E-737.591373145
GGGGTAT2750.036.9121256
CAGGGGT2800.036.252984
GCGGTAT1655.999209E-835.1544086
TATACCC850.002740734734.116543145
AGGTATC3250.033.4642877
Produced by FastQC (version 0.11.5)