FastQCFastQC Report
Sun 19 Mar 2023
SRR4896123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385083
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGA900.0144.91785
AGAGATC386100.0144.52372
AAGAGAT386500.0144.374131
GAGATCA115050.0144.225023
GAGATCG109650.0144.19093
AGATCAG37950.0143.390344
AGATCGT43650.0143.25784
AGATCTG48250.0143.115724
AGATCCG3900.0143.059894
GATCTAG15400.0143.035775
AGATCGA36600.0142.938054
GATCGAG10700.0142.886255
GATCGAT13850.0142.825135
GAGATCT151200.0142.425833
AGATCAT42850.0142.381324
GATCATG12850.0142.098395
GATCAGT13300.0141.648995
AGATCAA34400.0141.547624
GATCAGA9100.0140.936555
GATCAAG12200.0140.760335
Produced by FastQC (version 0.11.5)