FastQCFastQC Report
Sun 19 Mar 2023
SRR4896121_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896121_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012716
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT102520.5093614797119912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA2016050.0144.680972
TTCACGC2016300.0144.663021
CACGCAT734750.0144.320193
ACGCATG263650.0144.014824
ACGCAGT232350.0143.892644
CACGCAG656750.0143.586753
ACGCATA216750.0143.584554
ACGCATT251350.0143.45154
CACGCAA588850.0143.149783
ACGCAAT217900.0142.79354
CGCAGTA70750.0142.7715
CGCATAG71450.0142.589225
ACGCAGA193150.0142.445764
CGCATTG81850.0142.26595
ACGCAAG200950.0141.856744
CGCATGA65700.0141.833655
CGCATAT72000.0141.70135
ACGCAGG229350.0141.699194
CGCAATG67850.0141.077135
CGCATGG80850.0141.067935
Produced by FastQC (version 0.11.5)