FastQCFastQC Report
Sun 19 Mar 2023
SRR4896121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012716
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG379591.8859590722188326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136820.6797779716562098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA56720.28180826306344264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG44760.22238606937093958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT571350.099.0862661
GGTATCA580850.097.435878
AGTGGTA581600.096.853795
GTATCAA586200.096.638369
GTGGTAT585450.096.075866
TGGTATC587100.095.879947
AGCAGTG590050.095.823112
CAGTGGT598050.094.323084
GCAGTGG605700.093.083893
TCACGCA5000.040.603542
TTCACGC5350.037.9472351
GGGTATC6150.030.6515587
CACGCAT4000.027.189873
AGGGGTA7300.026.8174085
CGATAAG2456.44759E-826.631668145
ACCGTTC1751.256659E-424.856224145
AGGTATC7400.024.4941647
CGGTATC3102.4199835E-823.3879747
CATGGGG124450.023.26787225-29
ATGGGAT71550.023.00168825-29
Produced by FastQC (version 0.11.5)