FastQCFastQC Report
Sun 19 Mar 2023
SRR4896120_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896120_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659820
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA663000.0144.202712
GTGGTAG220500.0143.686363
GTGGTAA191650.0143.541153
TGGTGGT667350.0143.262761
GTGGTAT244200.0142.854953
TGGTAAG65500.0141.694344
TGGTAGT82500.0141.30154
TGGTAGG77800.0140.990864
TGGTATG89900.0140.46964
TGGTATA73300.0140.058854
TGGTAGA62400.0139.328934
TGGTAAT72450.0139.0024
GGTAGTA23900.0137.323535
GGTATAG24550.0137.229085
TGGTAAA58450.0134.985784
GGTATGG32450.0134.854975
GGTAAGC10400.0134.452425
GGTATGA22450.0133.929555
GGTAATG23350.0133.421635
GGTATTG29550.0133.133395
Produced by FastQC (version 0.11.5)