FastQCFastQC Report
Sun 19 Mar 2023
SRR4896120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659820
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG214853.2561910824164166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68111.0322512200297052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29680.4498196477827286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA20650.31296414173562487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18740.28401685308114333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA11350.17201661059076717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9740.14761601648934558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT9380.14215998302567365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG8510.1289745688217999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT8470.1283683428813919No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8400.1273074474856779No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8390.12715589100057592No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATTGCTGTTTTTGTTTT7830.11866872783486404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA6700.10154284501833832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT510150.0116.242241
AGCAGTG520950.0113.7263262
GGTATCA523700.0113.5496758
GTATCAA524100.0113.393869
AGTGGTA522900.0113.0110555
GTGGTAT523300.0112.860716
CAGTGGT524850.0112.701694
TGGTATC524550.0112.6332247
GCAGTGG532100.0111.1388553
AGGGGTA3350.067.088125
AGCGGTA1251.8189894E-1257.9987565
CCGCAAG3850.054.605083145
GCGGTAT1353.6379788E-1253.6984836
CAGGGGT4600.048.857654
GGGTATC4600.048.8539477
CGGTATC1551.4551915E-1146.7696467
AGGTATC3900.046.4698377
GGGGTAT5150.043.636536
GAGGTAT4350.041.6626176
AGAGGTA4850.037.3703355
Produced by FastQC (version 0.11.5)