FastQCFastQC Report
Sun 19 Mar 2023
SRR4896119_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896119_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458751
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT201774.398246543331786No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT36700.7999982561345915No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT22380.48784634801885995No Hit
TGGCTTCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT6990.1523702400648718No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGT6220.13558553550836946No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTC4950.10790167214894354No Hit
TGGCTTCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCCGC4930.1074657057968266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA459500.0144.678672
GCTTCAG141800.0144.506423
GCTTCAT191350.0144.309343
TGGCTTC461200.0144.145391
GCTTCAA117750.0143.930633
CTTCATG101650.0143.77474
CTTCAGA42400.0143.377184
CTTCAGT47800.0143.247774
CTTCAGG51700.0142.11224
TCATGTA55300.0141.508516
CTTCAAG41350.0140.709694
CTTCAAT44750.0139.895784
TTCAGAT13700.0139.626335
TTCATGT68200.0139.284335
CTTCATA41150.0138.576264
TTCAGTA14750.0138.529115
TTCAGAG12500.0137.959275
TTCAGTG15450.0137.41155
CTTCATT51900.0136.119784
TTCAGGA15700.0135.223435
Produced by FastQC (version 0.11.5)