FastQCFastQC Report
Sun 19 Mar 2023
SRR4896118_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896118_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534784
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAACAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5750.10752004547630446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA536400.0144.46161
GAACAAT198950.0144.033313
GGAACAA539350.0143.671462
GAACAAG186850.0143.511383
AACAATG72000.0140.278374
AACAAGT67600.0139.119634
ACAAGTG20600.0138.224725
ACAATGT26050.0137.675875
ACAATGG23400.0136.856935
CAATGTG9750.0136.733086
ACAATTG24050.0136.471985
ACAAGGT24700.0136.10735
ACAATAG21400.0136.104555
GAACAAA151400.0135.288333
ACAATGA19400.0134.823585
AACAAGG76450.0134.671894
CAATGAG6700.0134.093356
CAATATG8000.0134.039296
CAATAGG6800.0133.186896
CAATGTA8950.0132.764276
Produced by FastQC (version 0.11.5)