FastQCFastQC Report
Sun 19 Mar 2023
SRR4896118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534784
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG197923.700933460986118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34760.64998204882719No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27250.5095515198659646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14690.2746903422690282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGACCCGCTGGGCACA8730.1632434777405457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8620.16118657252273816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG6430.12023545955002393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT201250.082.605341
AGCAGTG207100.080.131942
GGTATCA208050.079.5843668
AGTGGTA208200.079.422565
GTGGTAT208050.079.37536
GTATCAA208550.079.281869
CAGTGGT209850.078.7709054
TGGTATC210900.078.3370367
GCAGTGG212300.077.827713
GCCGACG1504.397596E-528.997417145
AGGGGTA2553.0195224E-928.4288435
CGGTCTG3503.6379788E-1226.926174145
CATGGGG42550.023.8517225-29
ATGGGGG13500.022.23093825-29
GGGGTAT2953.8707185E-722.1166766
CGACGTA604.5614433E-721.74969185-89
CGACCTA1350.021.48117645-49
CCGATCG1150.021.43568100-104
TCGATTT757.783456E-921.265171135-139
TACGCTA555.2133128E-621.09218870-74
Produced by FastQC (version 0.11.5)