FastQCFastQC Report
Sun 19 Mar 2023
SRR4896117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151306
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAG2150.0144.892276
GATGCCT151.2316E-4144.892277
GATTGAG1000.0144.892277
GATTCGG253.7125574E-8144.892277
GATTAGG600.0144.892277
AGGCTAG151.2316E-4144.892278
ATATGCG100.00710072144.892269
GTAATAG100.00710072144.892269
ATCGGTA100.00710072144.892269
AGTCGTG100.00710072144.892268
GATAGCG400.0144.892267
TGAAGAG151850.0144.367461
GAAGAGA153000.0143.282352
AAGAGAT53700.0142.3293
AAGAGAG52200.0140.034773
GATTAGT900.0136.84277
AAGAGAA46850.0136.5423
GAGATTA5900.0133.841175
AGAGTTG1900.0133.453416
AGAGAGT19600.0131.955464
Produced by FastQC (version 0.11.5)