FastQCFastQC Report
Sun 19 Mar 2023
SRR4896117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151306
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG73234.839860944047163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28921.9113584391894571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9900.6543032001374698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3100.2048828202450663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGGCCTTTCCAAGATTGC2590.17117629175313603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2430.16060169457919712No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2360.15597530831559886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2220.1467225357884023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA2170.1434179741715464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACTGGGAGAGTCTCCTGA2020.1335042893209787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTCTACTAAAAATACAAA1650.10905053335624497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTACACCTGAAAGAAGA1620.10706779638613143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGTACATTCCTGATACT1590.10508505941601787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGATTAAAATAATTTGA1560.10310232244590432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGATGAGAACATTATCTG1540.10178049779916197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATAACCCTGAGATTCT1530.10111958547579078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG202.1247051E-6145.0145
TATTCGA100.0070849587145.0145
AAGCAGT127200.0111.485851
CGCACTG203.8596493E-4108.75145
AGCAGTG130550.0108.458442
GGTATCA131350.0108.184248
GTATCAA131250.0108.156199
AGTGGTA130550.0108.06975
TGGTATC131000.0107.919857
CAGTGGT130900.0107.7253654
GTGGTAT131050.0107.657386
GCAGTGG133900.0105.203513
TTGCGCT451.227816E-680.55556145
CACTAGA502.2967797E-672.5145
GGGGTAT851.29548425E-859.705886
GAACGTG651.0891899E-555.76923145
GTACAAT400.00604100554.375145
AGTGCTA400.00604100554.3755
ATACCAT604.9316965E-448.333332145
AGGGGTA1109.8843884E-846.1363685
Produced by FastQC (version 0.11.5)