FastQCFastQC Report
Sun 19 Mar 2023
SRR4896116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677822
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT116521.7190353809702252No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT20460.3018491580385411No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT14500.21392046879564253No Hit
TCTTCACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT12310.181611101439611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA681600.0144.102342
TCACATG112000.0144.004534
TTCACAG223450.0144.00233
TCTTCAC682100.0143.996691
TTCACAT262700.0143.200213
TCACAGG79650.0143.181854
TCACAGT79550.0143.179674
TCACAGA63500.0142.628174
CACATGT60200.0142.1425
TCACAAG63600.0142.062154
ACATGTA42200.0141.648036
TCACATA70500.0141.415924
CACAGAT22450.0140.391865
TTCACAA187850.0140.243163
TCACAAT68150.0139.381734
CACATGG27850.0139.186665
CACATAG22500.0139.113835
TCACATT80900.0138.90964
CACAGTG26250.0138.837785
CACATTG28650.0138.587785
Produced by FastQC (version 0.11.5)