FastQCFastQC Report
Sun 19 Mar 2023
SRR4896116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677822
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG413916.106470430289958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA63310.9340210261691122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54010.7968168634243208No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49200.7258542803272836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28380.41869399340829894No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10190.15033445358811015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG7060.10415713859980939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT560200.097.629981
GTATCAA573350.095.756069
GGTATCA573400.095.678918
AGTGGTA569450.095.598465
AGCAGTG572000.095.552562
GTGGTAT570950.095.365666
TGGTATC572850.095.163267
CAGTGGT574650.094.809094
GCAGTGG582700.093.4993063
AGGGGTA3350.051.9455835
GGGTATC4050.041.1739927
GGGGTAT4400.039.5465626
AGGTATC5050.035.8920947
CAGGGGT4950.035.1550944
AAATATA22100.034.772163145
GAGGTAT5450.033.2578126
AGAGGTA5800.031.253185
CATGCGG1255.1534915E-428.99867145
CGGTATC1555.4967975E-528.0653027
GCGGTAT1606.823642E-527.1882616
Produced by FastQC (version 0.11.5)