FastQCFastQC Report
Sun 19 Mar 2023
SRR4896115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652702
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8540.13084072057386067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA653650.0144.696492
CGTCTAG216800.0144.673143
CGTCTAT225550.0144.489473
CGTCTAA196750.0144.244233
GTCTAGA64550.0144.233614
TCCGTCT656800.0144.00251
GTCTAAG67350.0143.938874
GTCTAGT77050.0143.87274
GTCTAAT71250.0143.585114
GTCTAGG74200.0143.540024
GTCTATG81650.0143.221084
GTCTATA70150.0142.738114
TCTATAG23850.0141.869195
TCTAGAG21400.0140.844275
GTCTATT76000.0140.71244
TCTAGAT21050.0140.088325
CTAGTAG7350.0139.978266
GTCTAAA59450.0139.78844
CTAGATG7700.0139.261346
TCTAGTG25600.0138.963625
Produced by FastQC (version 0.11.5)