FastQCFastQC Report
Sun 19 Mar 2023
SRR4896115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652702
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG149372.2884869358451483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG72301.1077030559121928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51230.7848911141684872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38940.5965969155908822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21160.32419082521579523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA11480.1758842473287963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT206600.096.8279341
GGTATCA210100.095.17318
GTATCAA210900.094.804839
GTGGTAT210350.094.542996
TGGTATC211850.093.805147
AGCAGTG213600.093.62082
CAGTGGT213100.093.398144
AGTGGTA214900.092.5755
GCAGTGG216500.092.065343
GTCGTTC300.001935886572.49578145
AAATATA20900.047.17429145
CGGCTAT950.004727449330.524544145
AGGTATC2603.7143764E-927.8851327
AGCGGTA1050.007705825427.6195585
TTACTGA6900.027.317251145
AAACCGC14300.025.55252885-89
GAAACCG14900.025.01014785-89
CATGGGG60800.024.49192625-29
CGCAGAT15650.024.18070490-94
ATGGGGG22650.024.00596225-29
Produced by FastQC (version 0.11.5)