FastQCFastQC Report
Sun 19 Mar 2023
SRR4896114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205803
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT64873.152043459036068No Hit
ACATTGGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACCACT3940.19144521702793446No Hit
ACATTGGCGTTGATACCACTGCTTCCCATGTACCCTGCGTTGATACCACT2680.1302216197042803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG100.0070989206144.917139
CATGAGT151.2325906E-4144.881938
CGGTTAG400.0144.881938
GCTATCG100.0071040806144.881937
GCAGTCA202.1351843E-6144.881937
GCTGACG100.0071040806144.881937
CGGATTA100.0071040806144.881938
CTACGTG100.0071040806144.881938
CTAGTAG306.511982E-10144.881938
CATAGAG100.0071040806144.881938
GCTAGTA750.0144.881937
CGTAATC100.0071040806144.881938
CGTAAGC100.0071040806144.881938
CAATGTG151.2325906E-4144.881938
CGATGAG202.1351843E-6144.881938
CGGTATT151.2325906E-4144.881938
CTAGGTA202.1351843E-6144.881938
CGGAGTA253.718924E-8144.881938
CGATAAG100.0071040806144.881938
CGAAGTG253.718924E-8144.881938
Produced by FastQC (version 0.11.5)