FastQCFastQC Report
Sun 19 Mar 2023
SRR4896113_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896113_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551487
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT70280.27544721960174595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCA2553650.0144.79612
TATCAGC2553700.0144.793271
TCAGCAT903850.0144.488043
TCAGCAG858250.0144.287753
CAGCATG329750.0144.239754
TCAGCAA741400.0144.021793
CAGCAGT309300.0143.866714
CAGCATT302800.0143.485144
CAGCATA268600.0143.194414
CAGCAAT265000.0143.088934
AGCATGA83150.0142.65525
AGCATTG100200.0142.317585
AGCATGT121400.0142.175325
CAGCAAG264400.0142.125584
AGCATGG106600.0141.522255
AGCATAG92700.0141.325275
AGCAGTT93450.0141.121545
AGCAGGT100750.0140.965295
AGCAGTA92550.0140.849695
AGCAAGT85250.0140.841065
Produced by FastQC (version 0.11.5)