FastQCFastQC Report
Sun 19 Mar 2023
SRR4896113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551487
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG367061.438612072097565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA257151.007843661362962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC70510.27634865472565606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGTCGCGGTGGCTCA62920.2466012956366229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC62840.24628775298482808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA57430.2250844311572036No Hit
GGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGC56610.22187061897630678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA54000.2116412899615009No Hit
GAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCT45990.18024783195054492No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT45990.18024783195054492No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43090.16888191082298284No Hit
GCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTG41420.16233670796676605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGTCGCGGTGGCTCAT40160.15739841120099768No Hit
GACGACATCGGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACAT39050.15304800690734463No Hit
GCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTC38790.15202899328901148No Hit
TTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCT37600.14736504634356357No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCATGGTGGCTCA36850.1444255839829872No Hit
GTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCT36730.14395527000529496No Hit
GGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACATCCTGCGCGT36610.14348495602760275No Hit
GGACCCGGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGG35480.13905616607100096No Hit
AACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTT35340.13850746643036002No Hit
GGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACG33580.13160952809087406No Hit
CGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCG32750.12835652307850284No Hit
GAGCTGCACGCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCT32220.1262793030103622No Hit
TGTCCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTC31130.12200728437965781No Hit
CCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACG31110.12192889871670912No Hit
TGCACGCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCTGTCC29210.11448226073658223No Hit
CAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGCGCT28870.11314970446645427No Hit
TGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG28730.11260100482581334No Hit
CGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACA28690.11244423349991593No Hit
CGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCC27780.1088776858357499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG27490.10774109372299369No Hit
GACATCGGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACATCCT27350.10719239408235276No Hit
TGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCAC27000.10582064498075044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCTT26030.1020189403277383No Hit
CTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGC25760.10096073387793078No Hit
GTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGCG25670.10060799839466163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT503500.098.5732961
AGCAGTG518500.095.6917652
AGTGGTA517250.095.412785
GGTATCA522100.095.314258
GTGGTAT519500.094.981866
GTATCAA523150.094.8143549
CAGTGGT523450.094.468274
TGGTATC524900.094.0875857
GCAGTGG596250.083.019163
GCTATCC10000.053.64743145
ACGGCGC3700.047.026628
GACGGCG3700.047.026627
GCTATTC11750.046.891376145
TCGACGG3750.046.401425
GCGTAGA3100.044.4394572
CGCGTAG3200.043.0515671
CGTAGAC4550.041.432273
AGGGGTA4950.039.546665
GGGGTAT4800.039.2704936
GGCTATC12450.039.014202145
Produced by FastQC (version 0.11.5)