FastQCFastQC Report
Sun 19 Mar 2023
SRR4896112_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896112_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324079
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTCG202.134826E-6144.912357
TAGGATG324700.0144.622271
AGGATGA324950.0144.5112
GGATGAG92150.0143.968813
GGATGAT127250.0143.887453
GGATGAA101650.0143.272923
TGAGTAG4800.0138.874346
GATGAAT40150.0138.415664
GATGATA40750.0137.266664
GATGATT49500.0136.276174
ATGAGTA11550.0136.129795
GATGAAG32850.0136.089694
GATGAGA31100.0135.82624
ATGATTG15900.0135.342685
GATGATG43050.0135.318854
GATGAAA33000.0134.592834
GATGAGT34250.0134.546374
ATGAATG14500.0134.418725
TGATGAG5250.0133.871416
GATGAGG31900.0133.55564
Produced by FastQC (version 0.11.5)