FastQCFastQC Report
Sun 19 Mar 2023
SRR4896112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324079
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG114023.5182779507465773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23690.7309946031677461No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15090.46562720818072134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12220.37706855427226077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGGCCTTTCCAAGATTGC6690.2064311479608367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5590.1724888067415661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATTGCTGTTTTTGTTTT4560.14070643269079453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCCATCTCTA3710.11447825993044906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT3490.10768979168659493No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3430.10583839125645289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT251100.0117.656441
AGCAGTG257400.0114.709932
GTGGTAT257400.0114.4000556
GGTATCA258650.0114.323768
GTATCAA258350.0114.298559
CAGTGGT258000.0114.1902164
AGTGGTA258150.0114.151955
TGGTATC258600.0113.86927
GCAGTGG261350.0112.754273
CGGATAC203.8637884E-4108.74918145
AGGGGTA1650.070.313365
AGCGGTA451.1917787E-464.453915
GGGTATC2200.052.7350127
GCGGTAT701.6919488E-551.793326
GGGGTAT2450.047.3538936
CAGGGGT2500.046.4068154
CAGCGGT750.00148083638.6723444
AGCAGGG3000.038.6723442
CGGTATC800.002033121736.255327
TAGACTA1051.8510403E-434.523552145
Produced by FastQC (version 0.11.5)