FastQCFastQC Report
Sun 19 Mar 2023
SRR4896111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640916
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15940.2487065387663906No Hit
GTGTTCTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT7020.10953073413676674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG210150.0144.057563
TGTTCTA644800.0144.043052
GTTCTAA184250.0143.34653
GTGTTCT650350.0142.81381
GTTCTAT242450.0142.173
TTCTAGG75300.0140.58934
TTCTAGA59150.0140.26454
TTCTAGT78950.0140.238824
TTCTAAG64150.0140.175524
CTATAGG8650.0139.893456
CTATGAG7150.0139.852456
TCTAGTG24500.0139.5965
TCTAGAG18700.0139.494775
TCTATGG31400.0139.150475
TCTATAG25600.0138.975625
TTCTATG90500.0138.354144
TCTAGGG26950.0138.197885
CTAGTAG7200.0137.874866
TCTAGAT21150.0137.724985
TCTAAGT24000.0137.371675
Produced by FastQC (version 0.11.5)