SRR4896110_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR4896110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50189
Sequences flagged as poor quality	0
Sequence length	151
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	8608	17.15116858275718	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	1476	2.9408834605192373	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA	392	0.7810476399210983	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT	242	0.48217736954312695	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC	207	0.41244097312160033	No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	198	0.3945087568989221	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGACGTGGGGTTTCACCATG	140	0.27894558568610656	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCATGTTTATTGGAAGC	127	0.2530434955866823	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTCAGCGCCACCTGGG	118	0.23511127936400406	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC	116	0.2311263424256311	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	102	0.20323178385702045	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT	97	0.19326944151108807	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA	95	0.18928450457271515	No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	94	0.18729203610352865	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG	86	0.17135228835003685	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGGAAGCTGGGTATGGTGA	79	0.15740500906573154	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	78	0.15541254059654508	No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACTTGGCG	78	0.15541254059654508	TruSeq Adapter, Index 20 (96% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTGCCCAGTTGTCTGCCC	77	0.1534200721273586	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCTAAGTAGCTGAGA	76	0.15142760365817212	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGGACCTTGCCGAGTTCTC	75	0.14943513518898563	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGTACTTCTCAGCGGAGC	72	0.1434577297814262	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGACCCAATGCACGAAGCCA	71	0.14146526131223974	No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	68	0.13548785590468032	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGATGCAAGTCACTAA	66	0.13150291896630736	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT	66	0.13150291896630736	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTCTTGCTTCAA	65	0.12951045049712087	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGACTTGGTGTTGCTTGG	65	0.12951045049712087	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAGTTAAATTCTCAGAT	64	0.1275179820279344	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	64	0.1275179820279344	No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	64	0.1275179820279344	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	63	0.12552551355874791	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTTCATGCGTAGGTTTC	63	0.12552551355874791	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGGCTCACGCCTGTAATC	63	0.12552551355874791	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGATCACCTGAGGTTAGG	63	0.12552551355874791	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTACCTGAATGAGCAGGTG	62	0.12353304508956146	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCCTTAACGAGGAACAAT	62	0.12353304508956146	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCTTATTTTTCATAAGTAT	61	0.12154057662037498	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG	61	0.12154057662037498	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	60	0.1195481081511885	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTTTGGATCCCTTCTCTAGA	60	0.1195481081511885	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG	60	0.1195481081511885	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAATGATGGTGCTGCTAC	59	0.11755563968200203	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	58	0.11556317121281555	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATGAGGATTATGCGGGAC	58	0.11556317121281555	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC	58	0.11556317121281555	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTTCCTTAATGTACCT	57	0.11357070274362907	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCGTGGTATCAACGCAG	56	0.1115782342744426	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTTCTGAGTCATAACCAG	56	0.1115782342744426	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG	55	0.10958576580525613	No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	55	0.10958576580525613	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGGCTGGGCACAGTGGCTC	55	0.10958576580525613	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	54	0.10759329733606966	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC	54	0.10759329733606966	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGAGAAAAAAACAAAAAA	53	0.10560082886688318	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCCGAGACAGTCGGATC	53	0.10560082886688318	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG	53	0.10560082886688318	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACCGCAG	52	0.1036083603976967	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAATGTCACCCGAGTCGAC	52	0.1036083603976967	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG	52	0.1036083603976967	No Hit
ATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCA	52	0.1036083603976967	No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG	51	0.10161589192851023	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGGG	10	0.007072534	144.98903	145
GCCGTAA	10	0.007072534	144.98903	145
TCTCTAT	20	3.845602E-4	108.741776	145
TGCTAGA	30	1.5852129E-5	96.659355	145
TCTGGGT	55	4.0563464E-10	92.26575	145
AAAGGAC	35	3.4067518E-5	82.850876	145
AAAAGAC	30	0.0019255787	72.494514	145
AATATTC	30	0.0019255787	72.494514	145
ATGAATC	30	0.0019255787	72.494514	145
AAGCAGT	5835	0.0	61.187492	1
AGTGGTA	6015	0.0	59.29726	5
GTATCAA	6105	0.0	59.254322	9
GGTATCA	6165	0.0	58.67764	8
AGCAGTG	6090	0.0	58.62545	2
ACAAGCC	50	1.9925502E-4	57.995613	145
CAGTGGT	6195	0.0	57.397522	4
GTGGTAT	6230	0.0	57.250885	6
TGGTATC	6330	0.0	56.46098	7
GCAGTGG	6320	0.0	56.262287	3
CCTGGGA	100	2.6837024E-6	43.49671	145

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content