FastQCFastQC Report
Sun 19 Mar 2023
SRR4896109_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896109_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860966
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC64660.7510168810382756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG861400.0144.84021
TCGTAGA861650.0144.798172
CGTAGAG265650.0144.452223
CGTAGAA248300.0144.215553
CGTAGAT337350.0144.20713
GTAGATG110200.0143.009124
GTAGAAG89100.0142.31364
GTAGAGT92150.0141.770694
GTAGATT113550.0141.342474
GTAGAGG94850.0141.09634
GTAGAGA81050.0141.071764
GTAGATA116450.0140.99594
GTAGAAT93650.0140.892614
TAGATAG35200.0138.946375
TAGATTG37100.0137.689655
TAGATGA31900.0137.647385
CGTAGAC14100.0137.207613
TAGAGTG29900.0136.918875
GTAGAAA71150.0136.768834
TAGAGTA27200.0136.657845
Produced by FastQC (version 0.11.5)