FastQCFastQC Report
Sun 19 Mar 2023
SRR4896109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860966
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG325563.7813339899601144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87021.0107251622015272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA37480.4353249721824091No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27910.3241707570333788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA14410.16737014005198814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13730.15947203490033288No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA12320.1430950815711654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG12080.1403075150470518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT9260.10755360838871687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCACTGTATTTTAATATTT9220.10708901396803126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTTAGTTTGTGGCTTCAT8980.10430144744391764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT633500.0111.472591
GGTATCA640150.0110.955298
GTATCAA639850.0110.910219
AGCAGTG647850.0109.003452
GTGGTAT648550.0108.690896
AGTGGTA649300.0108.565345
TGGTATC650200.0108.470827
CAGTGGT656200.0107.445864
GCAGTGG660150.0106.759033
AGGGGTA4450.068.433735
CGGTATC1500.062.839557
AGCGGTA1600.058.912085
GGGTATC5100.058.290087
GGGGTAT5250.058.0057376
CGGTCTG1650.057.120228145
GCGGTAT1750.053.8624736
GTCGTAG1353.6379788E-1253.70591
TCGTAGA1457.2759576E-1250.0020452
AGGTATC4400.046.1409267
CAGGGGT7050.042.1672944
Produced by FastQC (version 0.11.5)