FastQCFastQC Report
Sun 19 Mar 2023
SRR4896108_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896108_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525978
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT159553.033396834088118No Hit
GTACGCAATGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC21320.40534014730654133No Hit
GTACGCAAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20410.38803904345809137No Hit
GTACGCAAGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA15880.301913768256467No Hit
GTACGCAAGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACCACT7520.14297175927510275No Hit
GTACGCAAATTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT6250.1188262626953979No Hit
GTACGCAAGTTGGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACT5910.11236211400476827No Hit
GTACGCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5740.10913003965945345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA526300.0144.780261
TACGCAA526700.0144.67032
ACGCAAT178550.0144.47093
CGCAATG70600.0144.363594
CGCAAGT94850.0144.036534
ACGCAAG202950.0144.019973
CGCAATT58250.0143.633014
CGCAATA48400.0143.379944
GCAAGAT13800.0143.301855
GCAATGT28400.0143.091165
ACGCAAA139350.0142.693313
GCAAGTT48200.0142.472025
CGCAAAT50450.0142.292084
GCAAGGT20800.0142.090525
CGCAAGG65250.0141.768144
GCAATTG20600.0141.711825
GCAATGA15600.0141.626175
CAAGTTG36550.0141.507376
GCAAGTG23050.0141.419685
GCAATGG22600.0141.030325
Produced by FastQC (version 0.11.5)