FastQCFastQC Report
Sun 19 Mar 2023
SRR4896107_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896107_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913923
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA915050.0144.726562
GGTGCGA915850.0144.600161
TGCGAAG309500.0144.580953
TGCGAAT329950.0144.491463
GCGAATG112600.0144.136544
GCGAATA101600.0143.767684
TGCGAAA262900.0143.723623
GCGAATT114350.0143.641454
GCGAAGT112850.0143.62464
GCGAAAT98850.0143.149554
CGAATGA31150.0142.350115
CGAAGTT37950.0142.235815
CGAATAG35850.0142.079245
CGAAGTG35500.0141.847235
CGAAGTA32150.0141.753595
CGAATAT33300.0141.42745
CGAAATG33800.0141.264545
CGAATGG38550.0141.14975
CGAATTG36200.0141.105835
CGAATGT37750.0141.070045
Produced by FastQC (version 0.11.5)