FastQCFastQC Report
Sun 19 Mar 2023
SRR4896107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913923
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG184702.020958001932329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55680.6092416976047216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG51090.5590186481793323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24490.2679656820104101No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23450.2565861675436552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16600.181634557834741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9730.10646411130915844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT430250.0115.468541
GTATCAA440250.0112.8663259
GGTATCA442000.0112.677828
AGCAGTG440450.0112.658672
GTGGTAT441050.0112.213646
AGTGGTA441550.0112.1748055
TGGTATC442900.0111.761297
CAGTGGT443300.0111.721744
GCAGTGG447150.0110.743583
GGGTATC4050.044.756097
GGGGTAT4250.042.6499256
CGGTCTG2450.041.426823145
AGGGGTA5050.040.2029235
AGGTATC4850.037.3736467
GTGCGAA2750.036.9156072
GAGGTAT5000.036.2524386
GTATCAC5850.034.6993879
AGAGGTA5400.033.568915
AAATATA26350.031.91516145
GGTGCGA3300.030.7596421
Produced by FastQC (version 0.11.5)