FastQCFastQC Report
Sun 19 Mar 2023
SRR4896106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476609
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC479250.0144.125921
GAGAACA479250.0144.125922
AGAACAT165900.0143.66063
GAACATG58300.0143.560064
AGAACAG169200.0143.171393
GAACAGT58150.0142.808844
GAACATA49400.0142.433614
GAACATT57450.0142.404624
GAACAGG62700.0140.766714
GAACAAT48000.0140.398324
AACAGAT15650.0139.370995
GAACAAG47950.0138.731254
AGAACAA139500.0138.538023
GAACAGA49400.0138.473054
AACATGT20450.0137.486045
AACAGTG18450.0137.464925
AACATTG19300.0137.41815
ACAGTTG7150.0135.8066
AACATGG19200.0135.491935
AACATGA16950.0135.094485
Produced by FastQC (version 0.11.5)