FastQCFastQC Report
Sun 19 Mar 2023
SRR4896106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476609
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG134532.822649173641287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37870.7945716509759572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17570.3686459970332075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14560.30549150351755844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9160.1921910832569255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGATGGGTGACCACCT6560.13763902905736147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAGAAGATGGGTGACCA6440.13512124194045855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT5900.12379119991439524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA5790.12148322839056754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGCCGGGCGCGGTGGCT5340.11204152670218144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4890.10259982501379537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT259350.0111.054481
AGTGGTA265800.0108.293675
AGCAGTG266100.0108.210182
GGTATCA268650.0108.0780948
GTATCAA269450.0107.730299
GTGGTAT268350.0107.226346
CAGTGGT269100.0107.030774
TGGTATC269700.0106.850897
GCAGTGG272400.0105.574423
TAGCCGA1250.063.797646145
GCGGTAT1152.4811015E-950.4329226
GGGGTAT2350.046.2748876
AGCGGTA1307.3323463E-944.618425
AGAGGTA2150.043.8401955
CGGTATC1351.0244548E-842.961387
AGGGGTA2600.041.829775
GGGTATC2600.041.825387
GAGGTAT2600.036.248666
AGGTATC2750.034.271467
ACGGAAT900.003626995732.22103145
Produced by FastQC (version 0.11.5)