FastQCFastQC Report
Sun 19 Mar 2023
SRR4896105_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896105_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750846
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGGTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT11000.14650141307272063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA751300.0144.823582
GCTCGGT752100.0144.669531
TCGGTAT269400.0144.543463
TCGGTAA216850.0144.519043
TCGGTAG253650.0144.491523
CGGTATG95700.0144.465734
CGGTATA83100.0144.135254
CGGTAGT92400.0143.743714
CGGTAAG73300.0143.634924
CGGTATT89900.0143.388614
CGGTAAT78850.0143.265884
CGGTAGA70350.0143.066044
CGGTAGG90550.0142.999484
CGGTAAA65650.0142.050314
TCGGTAC13350.0140.577853
GGTAATG26800.0137.079215
GGTATAG28800.0136.868915
GGTAGTA27850.0136.594275
GGTAGTG31050.0135.118675
GGTATTG36200.0134.711565
Produced by FastQC (version 0.11.5)