FastQCFastQC Report
Sun 19 Mar 2023
SRR4896105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750846
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG142181.8935973555163108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG123401.643479488470339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC68280.909374225873215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32750.4361746616483274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16010.21322614757220523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15350.20443606278784196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14510.19324868215319788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT7760.10335008776766474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT235650.0108.581481
GTATCAA238800.0107.28679
GGTATCA239000.0107.25768
GTGGTAT238150.0107.0011446
AGCAGTG239500.0106.738082
TGGTATC240050.0106.365627
CAGTGGT240750.0105.9730764
GCAGTGG243150.0104.837623
AGTGGTA250500.0101.732625
AAATATA30800.062.8455145
TCGGTAT400.00605004954.3755723
CCGCAAG8650.042.74327145
CTCGGTA855.339884E-542.647512
ATCGCAT951.0289852E-438.155758145
GCGCTAT800.002036852836.24797145
AAAATAT45400.030.978441145
CGGTCTG1705.8448495E-729.851269145
AGGGGTA2951.4551915E-1129.4918375
CAGGGGT3650.027.8085144
GCTCGGT1409.987768E-425.8948551
Produced by FastQC (version 0.11.5)