FastQCFastQC Report
Sun 19 Mar 2023
SRR4896104_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896104_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756420
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG1757000.0144.86571
CTAACGA1757150.0144.853332
TAACGAG626050.0144.556413
TAACGAT617650.0144.53983
TAACGAA485600.0144.004993
AACGATG223850.0143.652824
AACGAGT213450.0143.421574
ACGATAG63300.0142.740345
AACGATA186900.0142.511574
AACGATT209850.0141.945774
ACGATGT72150.0141.90245
ACGAGTA64400.0141.764865
CGATATG22950.0141.7586
ACGAGTT62850.0141.687185
ACGAGGT73050.0141.542775
ACGATGA61900.0141.520575
AACGAGG235800.0141.166344
AACGAGA180150.0141.054024
ACGAGAT59400.0140.889775
ACGAAGT63050.0140.778055
Produced by FastQC (version 0.11.5)