FastQCFastQC Report
Sun 19 Mar 2023
SRR4896104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756420
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG247651.4099702804568384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109620.6241104063948258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG67700.3854431172498605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC44560.25369786269798794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32510.18509240386695666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20990.1195044465446761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT665850.0115.838861
GGTATCA672300.0114.840598
GTATCAA675300.0114.2347649
GTGGTAT673350.0113.907796
AGTGGTA674700.0113.8120655
TGGTATC675700.0113.651127
AGCAGTG679100.0113.549922
CAGTGGT680500.0112.9539954
GCAGTGG687200.0111.94773
CTAACGA2350.052.457182
GCTAACG2600.047.411871
AGCGGTA2150.047.2119145
GCGGTAT2150.047.210576
CGGTATC2150.047.210577
AGGGGTA6150.042.4413765
GGGTATC6800.039.4495937
TAACGAT2051.1374141E-831.8355643
GGGGTAT8700.030.8341626
GAGGTAT6700.030.2993226
AGGTATC6500.029.000787
Produced by FastQC (version 0.11.5)