FastQCFastQC Report
Sun 19 Mar 2023
SRR4896102_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896102_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731030
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA731650.0144.781592
GCGAGTA731650.0144.781591
GAGTAAG252250.0144.220953
GAGTAAT269550.0143.512563
GAGTAAA203450.0142.809143
AGTAATG98100.0141.512834
AGTAAGG92450.0140.678224
AGTAAGT95850.0139.543294
GTAATGG35550.0139.40745
AGTAAAG77800.0139.043124
AGTAAGA70550.0137.92674
AGTAATA80400.0137.520614
AGTAATT97300.0137.314824
GTAAGTA29000.0135.91595
GAGTAAC13350.0135.683843
AGTAAAT80100.0135.412464
GTAATTG34950.0135.166725
GTAATAG26750.0135.159355
GTAAGTG30150.0134.817085
GTAAGGT29600.0133.895195
Produced by FastQC (version 0.11.5)