FastQCFastQC Report
Sun 19 Mar 2023
SRR4896101_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896101_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770825
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT1774550.0144.600071
CACATAG625700.0144.547323
CCACATA1778950.0144.242422
ACATAGG225950.0143.367114
CACATAT611000.0143.056413
CATAGAG62900.0142.372165
ACATAGT219650.0142.168474
CATATGG77700.0142.015625
ACATAGA184900.0141.96744
ACATATG217750.0141.944954
ACATAAG183200.0141.623754
CATAGTG72600.0141.413365
CACATAA517750.0141.351843
CATAGGA62850.0141.332635
CATAAGG62400.0140.84245
CATAAGT59250.0140.504075
CATAGGT71100.0140.116845
CATATAG64000.0139.35915
CATAGGG75000.0139.303245
CATAGTA68450.0138.661225
Produced by FastQC (version 0.11.5)