FastQCFastQC Report
Sun 19 Mar 2023
SRR4896101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770825
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG229661.2969096325159177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95840.5412166645490096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG81980.46294806093205143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38270.21611395818333262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32420.18307850860474637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT550950.0114.5165561
GGTATCA559150.0113.02878
GTGGTAT556400.0112.753396
GTATCAA562950.0112.330329
TGGTATC559500.0112.180497
AGTGGTA561650.0111.712045
AGCAGTG565150.0111.440222
CAGTGGT566550.0110.8320544
GCAGTGG575100.0109.2221453
CGGTATC2650.043.7747967
AGGGGTA5400.041.6262255
AAATATA42400.035.73398145
GCGGTAT3400.034.118596
CACGAAC1301.6529477E-533.469082
TGCGCCC3750.032.863865145
CACGATA900.003625980632.2276651
GTGCCGG8500.031.556131145
CGGTCTG3450.031.51905145
GGGTATC7150.031.4344377
AGCGGTA4000.030.8168355
Produced by FastQC (version 0.11.5)