FastQCFastQC Report
Sun 19 Mar 2023
SRR4896100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432085
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG299876.940069662219239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43921.0164666674381198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43371.0037376905007116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGGAAGATTTATAGGTAG26630.6163139197148709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13050.3020239073330479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC10780.24948794797319973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT7890.18260296006572782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7810.18075147251119572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGTGGGAAGATTTATAGG7630.17658562551349852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC7620.176354189569182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7400.17126259879421873No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6460.14950762002846663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5730.13261279609336127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAGATTTATAGGTAGAG5510.127521205318398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA4380.10136894361063217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT266550.079.29151
AGCAGTG276800.076.311762
AGTGGTA276750.076.1945345
GTATCAA279750.075.541399
GGTATCA281000.075.446278
GTGGTAT280250.075.208376
TGGTATC281400.074.978327
CAGTGGT282250.074.7097854
GCAGTGG293600.071.8216553
CCGCAAG2450.062.139782145
GTGAGTA450.00963734348.330944145
TAGGGGT855.337996E-542.64495145
GCTATTC2900.039.99802145
CGGTCTG1105.259146E-639.543495145
GGCTATC3250.037.9212145
AGGGGTA1354.9556E-737.5994345
CACGGGA1553.4640834E-837.417503145
TATCCGC1406.600603E-736.248207145
CGTGCTC1854.1727617E-935.268524145
CACGGGC850.00274093534.11596145
Produced by FastQC (version 0.11.5)