FastQCFastQC Report
Sun 19 Mar 2023
SRR4896099_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896099_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916382
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAA917100.0144.766392
GAGCTGA917150.0144.758481
GCTGAAT329600.0144.467293
GCTGAAG312700.0144.398383
CTGAATG109400.0144.156464
GCTGAAA264150.0144.089573
CTGAAGT111900.0143.978534
CTGAATA107450.0143.131964
CTGAAGG110450.0143.048394
CTGAAGA90300.0142.317694
CTGAATT112700.0142.31374
CTGAAAG96950.0142.19494
CTGAAAT97000.0141.598834
TGAAGTG38650.0136.2635
GCTGAAC14300.0135.259663
TGAATAG37550.0135.238745
TGAATTG41650.0134.796845
TGAATGG38200.0134.454685
TGAATGT39950.0134.367575
CTGAAAA76350.0134.352974
Produced by FastQC (version 0.11.5)