FastQCFastQC Report
Sun 19 Mar 2023
SRR4896098_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896098_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856359
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT856800.0144.838351
ACTAGTA856950.0144.8132
CTAGTAG293750.0144.618473
CTAGTAT297750.0144.281773
CTAGTAA253550.0143.971423
CTAGTAC15500.0138.83743
TAGTAAG92900.0138.050924
TAGTAGA90900.0137.660774
TAGTATG109050.0137.539174
TAGTAGG108300.0137.086694
TAGTATA98400.0136.44644
TAGTAGT108600.0135.640474
AGTATGG39100.0135.09295
TAGTAAT94400.0134.629234
AGTATAG36150.0134.491855
AGTAGTG36050.0133.658985
AGTAGTA31550.0132.742575
AGTAAGG32250.0132.332755
AGTAGGT39200.0131.421165
AGTAATG34100.0131.315465
Produced by FastQC (version 0.11.5)